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Autoclave
Process |
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Bacterial spores which tends to be the most persistent, can
survive for long periods in adverse conditions. |
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Autoclave
Power/fuel |
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Steam is provided to the chamber from external source e.g.,
steam generator/boiler. |
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Autoclave
Process |
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Autoclave is a vessel in
which steam is held under pressure for the purpose of
sterilization. |
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Sterilization |
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Sterilization means
complete destruction or removal of all the micro
organisms. Including the most resistant bacterial
spores. The term is distinct from disinfection which
refers to killing the most harmful micro organisms
without achieving sterility |
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Types of Organisms & their
susceptibility to sterilization |
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The types of organisms
involved in sterilization process fall broadly into
three groups |
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Vegetative
Organisms |
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Include algae, bacteria and fungi. |
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Viruses |
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Bacterial
Spores |
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Bacterial spores which tends to be the most persistent,
can survive for long periods in adverse conditions. Some
pathological micro organisms are more dangerous then
others and have been categorized as Type A, B and C in
relation to their pathogenicity. Type A, B and C
organisms require special feature for sterilization but
most autoclaves can effectively be used for Type C
organisms without modification. |
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Applications |
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Media preparation. |
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Sterilization of fluids
in sealed containers. |
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Provision of safe
processing. |
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Quality control,
research and development, microbiology, pathology
labs. etc. |
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Industrial/
pharmaceutical production and injectable section. |
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Sterilization of
ampules, sterile packing of foods etc |
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Hospitals, laboratories
and surgical sections |
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