TECHNOLOGY INTERNATIONAL PAKISTAN

Autoclave Process

Bacterial spores which tends to be the most persistent, can survive for long periods in adverse conditions.

Autoclave Power/fuel

Steam is provided to the chamber from external source e.g., steam generator/boiler.

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Autoclave Typical Operation Cycle

		3 minutes at 134 ºC in saturated steam
		10 minutes at 126 ºC in saturated steam.
		15 minutes at 121 ºC in saturated steam.
		30 minutes at 115 ºC in saturated steam

To ensure that the load reaches the necessary temperature for the set period, the setting of the temperature controller should be some what higher than that actually required due to fluctuation of temperature within the chamber. The correct temperature / pressure correction for sterilization is shown in the table.
Type of Autoclaves
		Steam operated.
		Electrically heated.
		Horizontal (Front Loading).
		Vertical (Top Loading).
		Single door or double door.
		Round or square shape chamber.